Hello again,
have another question.
We're thinking of doing some gene expression analyses for a species on which we have no sequence data and the closest sequenced relative is 100+ million years away.
I'm thinking it may be possible to do everything in one shot:
1. RNAseq (using Illumina) on our 2 conditions of interest
2. Assembly of the RNAseq data to get good gene models -> annotate
3. "classic" RNAseq analysis where to identify differential expression
The alternative would be to first perform some 454 of a normalized library to get a good overview of the transcriptome.
Any experience with this? Do you think it's feasible?
Kind regards,
Yannick
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yannick . wurm @ unil . ch
Ant Genomics, Ecology & Evolution @ Lausanne
http://www.unil.ch/dee/page28685_fr.html
