abi autosequencing problems

Karen Lynn Hershberger khershbe at ezinfo.ucs.indiana.edu
Wed Apr 10 01:57:19 EST 1996


Hi All!

After 3 months of no problems, I have two sets of ABI 36 inch  plates that 
are massively fluorescing red or green.  I have tried cleaning repeatedly 
with Alconox, soaking in 3M HCl, and soaking in 10% 
KOH and EtOH, all of which takes some of it off but not everything.  The 
ABI guys ahve been out twice to check the machine, and says its fine.  
They suggested that it was the MeOH that I use to clean the plates that 
caused the fluorescence, but I have been using all along without a problem.  
Any ideas on how to clean the plates and what may have caused the problem 
in the first place (I don't want to go through this again!).

Thanks for any help! 

Karen Hershberger
Indiana University, Pace Lab




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