to PPT or not to PPT

Shaun Tyler styler at hpb.hwc.ca
Thu Aug 1 07:09:45 EST 1996


Our experience with EtOH ppt has been less than pleasing and we  have=20
reverted back to column purification.  We didn=92t have any problems with=
=20
T-Blobs but the first 50-60 bp were virtually unusable.  If you can live=20
with this than go with the EtOH ppt if you=92re doing only a few samples. =
=20
For a full run we have found that both methods take about the same amount=
=20
of time and the extra usable data aquired with the column method is worth=
=20
the extra expence.

Hope this helps :-)

=20
Shaun Tyler
DNA Core Facility
Laboratory Centre for Disease Control
Health Canada

Ph#:  (613) 941-6441
FAX#: (613) 957-1358

E-mail:  styler at hpb.hwc.ca




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