TAMRA BLOB USING DYEPRIMER CHEMISTRY

Sandra Hageman shag at nki.nl
Thu Aug 8 05:02:11 EST 1996


During sequencing of PCR products very often a so-called TAMRA blob 
appears at approx. 180-200 bases. On the gel image you can see it as a 
yellow haze. It obscures 6 to 10 bases. Very inconvenient. Does anybody 
else experience such blobs ? It seems to be independent of the template 
sequence, but dependent on the size of the PCR product. Shorter PCR 
product  get the blob somewhat earlier. 





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