More 377 Seq Problems

Matthew Oughton Matthew_Oughton_at_NCOT221 at isdtcp3.hwc.ca
Thu Aug 22 04:01:39 EST 1996


> Hi ,
> 
> Has anyone had problems with fried 36 well combs? We have lost three
> combs
> in 3 weeks, seems to be due to arcing to the back plate and happens
> 2/3 of
> the way through the run. 2-3 teeth are burnt off so the comb is
> wrecked.
> 
> We also lost a gel run today due to a -192 gel error, the gel file is
> corupted and the data is not able to be recovered has anyone else had
> this
> error?
> 
> Lastly anyone have any news on the 48 and 64 well upgrades?
> I hear that they are due out in September sometime ( in Australia at
> least) .
> 
> I am sceptical of the tracking ability of the software to handle 64
> (let
> alone 36 wells on a 373) well runs but I suppose we have to wait to
> see if
> the new software is any better. Anyone heard of any prices for the
> upgrade
> yet?
> 
> thanks.
> 
> Macky Edmundson - Queensland Institute of Medical Research
> 
> Socrates - To Be is To Do
> Jean Paul Sartre - To Do is To Be
> Frank Sinatra - Do Be Do Be Do
> 
> email mackyE at qimr.edu.au (Australia)


Hi Macky,
The problem with the melted combs (i.e. arcing) is a familiar one. This 
problem occurred 3 times and every time the buffer in the buffer chamber 
was below the glass plate. The 377 that we own is only 4 months old. It 
was discovered that our upper chamber leaked which was evident by the 
buildup of crystal on the bottom of the cooling plate as well as an 
elevated level of buffer in the bottom chamber. After calling ABI, it 
was evident that the problem is the white gasket in the upper chamber. 
With some further discussions the tecnical specialist (1800-831-6844) 
sent me a replacement 36 comb, a new gasket and some gasket silicone. I, 
then removed the old gasket and glue, filled the canal with gasket 
silicone, When almost curred the new gasket was installed. After 24 Hr. 
you can now use it. I have to date ran 5 separate sequence runs and the 
problem is now gone.

Hope this helps.

Robert Vogrig
DNA Core
Health Canada
Ottawa, Ontario, Canada
Email:Robert_Vogrig_at_NCOT221 at isdtcp3.hwc.ca




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