Band compressions in autosequencing

Alexander Kraev kraev at bc.biol.ethz.ch
Sun Aug 25 11:15:42 EST 1996


Dear fellow autosequencers,
I run a LI-COR 4000L and have recently come across a
cluster of band compressions in a clone, that could not be
resolved despite the use of deaza-G and running gel at 50 C.
My questions are : is anyone using formamide-containing
gels in automated sequencing and is, say, 20-30% formamide
in the gel compatible with all these fluorescent dyes,
particularly in ABI machines? I am interested in modifying
the gel since in my case the polymerase has no problem
reading through problematic region, so that there is a true
compression, not a BAFL.
-- 
Alexander Kraev, PhD                     Internet:   
kraev at bc.biol.ethz.ch
> Lab.of Biochemistry III                            Phone:   0041-1-632-31-47
> Swiss Federal Inst. Of Technology           FAX:      0041-1-632-12-13
> Universitaetsstr. 16                    Home Page: http://www.bc.biol.ethz.ch/BiochemistryIII/                                 
> CH-8092 Zurich                                     /Sasha/kraev.html





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