Colonic Irritation

Michele M. Godlevski mam7746 at glaxo.com
Tue Aug 27 04:18:01 EST 1996


In article <4vkneq$5vm at net.bio.net>, David Barrass
<Barrass at pplros.demon.co.uk> wrote:

> A cautionary tale:
> 
> A recent 377 run gave a good gel, but the first 10 samples were absent
> from the
> analysed data, and the error said File Not Found. The only distinguishing
> feature of these samples was that all the sample names had colons in them.
> Consultation with ABI revealed a bizarre bug, where if the sample name in
> the Sample 
> Sheet has a colon in it, the software is unable to recognize it, and the
> data 
> CANNOT be recovered!
> The only solution is to rerun the samples with a different name.  Future
> Sequence
> Analysis versions will have post-run editable sample sheets, but until
> then, the
> moral is to never put any colons in sample names.  You have been
> warned......
> 
> 
> Anthony.




Anthony,



Just a note...you should have been able to actually recover lanes that
have either not been named or been named improperly by moving the tracking
lines from the lanes that worked to the lanes that do not have names.  For
instance if sample 1 worked and sample 2 was not named, you could move the
line from sample 1 to lane 2 and regenerate sample files (from modified
lanes).  What you will get is a file called sample1.1 in the analyzed
data. You then just double click on that Seq. Analysis file and change the
name to what you want it to be.  As long as you get a gel file image and
at least one lane that works, you should be able to do this.  We routinely
use this techinique to put samples in lanes 0 and 37 to stretch our
current capacity from 34 samples and 2 controls per gel to 36 samples and
2 controls per gel.  



Michele Godlevski
Glaxo Wellcome
Sequencing Facility
Research Triangle Park, North Carolina




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