T3/T7 primers for sequencing

Michael C. Gorry mcgorry at med.pitt.edu
Thu Aug 29 16:51:50 EST 1996

I have been trying to use the T3 and T7 primers for sequencing
clones.  The results have been terrible.  When using internal
primers, I have been obtaining good reads.  After analyzing
the standard T7 and T3 primers on Oligo, I have come to realize
just how bad they are.  I am assuming that there are people who 
use them all of the time with good results. Can anyone offer any
suggestions on how to improve the reads?  I am using the ABI taq-FS
terminator kits. What should be done to optimize the results?

Thanks in advance for any help.


Michael Gorry
mcgorry at med.pitt.edu

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