T3 and T7 primers

Ken Taylor KTAYLO at scri.sari.ac.uk
Fri Aug 30 15:05:45 EST 1996


Hi all,
      to answer Michael Gorrie's query about T3 and T7 primers.
I don't know what sequence anyone else is using, but the following 
are the sequences I made and use.

T3  ATT AAC CCT CAC TAA AGG GA

T7  TAA TAC GAC TCA CTA TAG GG

These work fine in my Taq FS Dye Terminator reactions, using the 
cycles as listed in the protocol booklet.
I think they are a little longer than some published sequences.

Also, thanks to all who replied to my query about IBM compatible 
software.  I now have a copy of ABIView and think it will help some 
of my "customers" out.  Other recommendations such as GeneSkipper and 
Staden ( for Unix ) will probably prove too expensive for my budget.

Happy sequencing,  Ken.
   
   








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