T3 and T7 primers
KTAYLO at scri.sari.ac.uk
Fri Aug 30 15:05:45 EST 1996
to answer Michael Gorrie's query about T3 and T7 primers.
I don't know what sequence anyone else is using, but the following
are the sequences I made and use.
T3 ATT AAC CCT CAC TAA AGG GA
T7 TAA TAC GAC TCA CTA TAG GG
These work fine in my Taq FS Dye Terminator reactions, using the
cycles as listed in the protocol booklet.
I think they are a little longer than some published sequences.
Also, thanks to all who replied to my query about IBM compatible
software. I now have a copy of ABIView and think it will help some
of my "customers" out. Other recommendations such as GeneSkipper and
Staden ( for Unix ) will probably prove too expensive for my budget.
Happy sequencing, Ken.
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