jun at nih.go.jp
Fri Aug 30 03:31:45 EST 1996
In article <502am2$dqj at net.bio.net> Sensational Gravity Boy <ed at bio02.bio.uottawa.ca> writes:
> We ran a gel last night (on a 373) and although the gel file looks fine,
> the samples have a spacing of -12 !!! yes, minus 12. As a result, the
> chromatograms look horrid and there are stacked bases that are
> not been called, and essentially the 30 samples we ran are a total waste.
If you still have the data file (possibly named GelFile),
try "Adaptive" or "SemiAdaptive" basecaller.
This may clear the problem of some samples.
ISHIKAWA Jun, Ph.D. National Institute of Health
(jun at nih.go.jp) Dept. of Bioactive Molecules
More information about the Autoseq