Sequencing through GC rich regions

Brenda Brenda
Tue Dec 10 10:22:18 EST 1996


One of my clients is having problems sequencing through a very GC rich
region. They have been using the Prism Dye Terminator FS kit and have
tried adding 5% DMSO, increasing the denaturation temperature, and
linearising the DNA to read through the secondary structure(we think)
but the problem remains!
Should they try dye labeled primers with Taq or maybe Sequenase or can
anyone suggest anything else.

Thanks
Brenda



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