sequencing through a-t rich regions

Tom Tom
Fri Dec 13 20:07:04 EST 1996


I have been trying to sequence a PCR fragment through an ITS region. It
is about 80% A-T with dinucletied repeats, polymorhich regions etc.Most
have suggested it is a PCR issue caused by stuttering of the Taq. Others
have stated it is a sequencing issue.Any ideas? I am trying to avoid
cloning the prodeuct because this is the beginning of a large field
study.



More information about the Autoseq mailing list