sequencing through a-t rich regions

Anthony Anthony
Tue Dec 17 10:41:11 EST 1996


A PCR stutter should give multiple bands on a LMW agarose gel (eg 4%
Metaphor),
whereas a sequencing stutter will obviously only appear in the trace.  If
it
is a sequencing stutter, using Sequenase rather than Taq FS may well give
better 
reads, in conjunction with terminators.

Hope this helps,
		Anthony.



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