that pesky fluorescent front...
ed at bio02.bio.uottawa.ca
Mon Jul 8 12:37:59 EST 1996
to anyone who's had that buffer front-like fluorescent front...
any of you still need mineral oil overlays on your reactions ?
we've sort of noticed that when our samples are made on thermal cyclers
that don't require mineral oil, we've had no problems. When we remove the
mineral oil (either by extraction or with that handy parafilm method), the
problem has not been there...could mineral oil be part of the problem ?
I don't see why it should, and I hate perpetuating folklore but unless we
come up with a reasonable explanation, this will have to become one of
those "we don't know why, but it seems to be that way..." type of thing...
thanks for your replies,
University of Ottawa
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