Loss of Resolution

daemon at net.bio.net daemon at net.bio.net
Thu Jul 11 05:06:04 EST 1996


Dear All,

Has anyone else noticed a massive drop in sequence quality, using a 48 cm
gel on an ABI 377, after the first 550 bases?  Generally I get good
sequence over the first 450  - 550 bases, but past this point the peaks
become extended and indistinct.  Can anyone offer any suggestions for
improving the length of readable sequence?  The runs are at 2700V, 4.25%
acrylamide with dye terminating chemistry and the cycle sequencing is done
on a MJ Research DNA engine.

Any possible suggestions would be greatly appreciated,
Thanks in advance

Trish Russell






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