green haze

David F. Bishop bishop at msvax.mssm.edu
Mon Jul 22 15:42:19 EST 1996


>I am wondering if anyone has seen a green haze on their gel images.
>
>I have been running an ABI 377 autosequencer for about 8 months.  This is
>the second set of gel plates in which this background haze has occurred.
>It appears to be a build up of some kind and seems to become noticeable
>after about 4 months worth of runs on each set of plates.  I have tried the
>3N HClwash recommended by ABI on the first set of plates without any
>noticeable improvement.
>
>The haze does not seem to be affecting data results at the moment but suspect
>the background will get worse.
>
>
>Would appreciate any advice on solving this problem.
>
>
>I am also curious to know how long people use a set of plates before
>they need to be trashed.  Someone has told me 2 years.
>
>
>                                                                John Augliera
>                                                                auggie at mit.edu

We saw some green haze early on when our plates were in use only a couple
of months, but haven't seen any since we switched to desalting by spin
columns.  We clean each time with Alconox powder sprinkled on the wet
plates from a plastic pizza condiment shaker, followed by scrubbing with a
paper towel and rinsing with copious tap water and then distilled water.
We have not used any other methods to clean the plates to date and they are
about 10 months old.



David F. Bishop, Ph.D.                   | EMail:bishop at msvax.mssm.edu
Dept. of Human Genetics, Box 1203        |
Mount Sinai School of Medicine           | Phone: (212) 241-6946
5th Avenue and 100th Street, NY,NY 10029 | FAX:   (212) 360-1809





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