Read length on ABI 377

Rick Nicholson R.Nicholson at arnie.cfi.unsw.EDU.AU
Mon Jul 29 03:16:58 EST 1996


We are wondering how well other people are doing in obtaining the 
promised read length of 700 bp sequence with the promised accuracy of 
greater than 98.5% using the 48 cm gels on the ABI 377.  Is it 
possible?  If so what are the tricks?
   We actually get better (500-550bp) than promised sequence (450) 
from the short gels but when we switched to the long gels we only get 
the same length of accurate (99%) sequence (500-550).  
Does anyone else bother running the 48 cm gels?
   All comments greatly appreciated.  ABI has been of no help to us in 
this.
-- 
Richard C. Nicholson, BSc PhD
Senior Research Fellow
Centre for Immunology
St. Vincent's Hospital
Sydney, Australia 2010					email:R.Nicholson at cfi.unsw.edu.au




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