Read length on ABI 377
R.Nicholson at arnie.cfi.unsw.EDU.AU
Mon Jul 29 03:16:58 EST 1996
We are wondering how well other people are doing in obtaining the
promised read length of 700 bp sequence with the promised accuracy of
greater than 98.5% using the 48 cm gels on the ABI 377. Is it
possible? If so what are the tricks?
We actually get better (500-550bp) than promised sequence (450)
from the short gels but when we switched to the long gels we only get
the same length of accurate (99%) sequence (500-550).
Does anyone else bother running the 48 cm gels?
All comments greatly appreciated. ABI has been of no help to us in
Richard C. Nicholson, BSc PhD
Senior Research Fellow
Centre for Immunology
St. Vincent's Hospital
Sydney, Australia 2010 email:R.Nicholson at cfi.unsw.edu.au
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