to PPT or not to PPT
Thomson at bms.com
Tue Jul 30 16:25:16 EST 1996
We have been using the FS enzyme for nearly a year, but have continued to use
the centrisep columns for purification. Now, in order to cut costs and to
prepare for future automation of the facility, we are considering ethanol
There have been numerous communications concerning "T-blobs" and "loss of an 80
base front" which have us concerned that the switch to EtOH ppt's will decrease
the quality and reliability of the sequencing facility.
I would appreciate hearing the experience, good and bad, others in the newsgroup
have had with this method. What protocols do you use? Has anyone managed to
automate the dye-terminator reaction from A to Z.
Thanks for your input in advance.
Thomson at BMS.com
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