PPT or not to PPT
ballard at fmppr.fmnh.org
Tue Jul 30 17:56:04 EST 1996
Here is a really simple protocol that we use at The Field Museum in Chicago
for precipating 10=B5l cycle sequencing reactions-we do this in 96--plate
format. We don't get dye "blobs" and we don't loose the small fragments.
1. Add 40 =B5l ddH2O, 5 =B5l 3 M NaOAc, 125 =B5l Cold EtOH. Let sit 1=
at -20=B0C (I am not sure how important this 1 hour at -20=B0C actually is-=
have used 30min at -20=B0C without a problem).
2. Spin for 15 min. Aspirate off the liquid.
3. Add 200=B5l 70% ethanol.
4. Spin for 5 min. Aspirate the liquid.
5. Dry in the speedvac.
Hope this helps:
More information about the Autoseq