PPT or not to PPT

Bill Ballard ballard at fmppr.fmnh.org
Tue Jul 30 17:56:04 EST 1996


Hey:

Here is a really simple protocol that we use at The Field Museum in Chicago
for precipating 10=B5l cycle sequencing reactions-we do this in 96--plate
format. We don't get dye "blobs" and we don't loose the small fragments.

1.      Add 40 =B5l ddH2O, 5 =B5l 3 M NaOAc, 125 =B5l Cold EtOH.  Let sit 1=
 Hour
at -20=B0C (I am not sure how important this 1 hour at -20=B0C actually is-=
-I
have used 30min at -20=B0C without a problem).
2.      Spin for 15 min. Aspirate off the liquid.
3.      Add 200=B5l  70% ethanol.
4.      Spin for 5 min. Aspirate the liquid.
5.      Dry in the speedvac.

Hope this helps:

Cheers

Bill Ballard








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