sequencing strategy 2

jea at ukrv.de jea at ukrv.de
Tue Jun 18 08:32:38 EST 1996


I want to know if someone can help me to decide which sequencing strategy
is the best one. I want to start sequencing on a ABI377. I want to
sequence PCR-Products after cloning them in pUC18 (sureClone Kit,
Pharmacia). My question is: is it better to sequence plasmid DNA with the
cycle sequencing Kit or is it better to do solid phase sequencing
(Dynabeads) and use Sequenase? 

i hope someone can help me. 

bye 

Jeanette




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