George F. Mayhew
mayhew at genetics.wisc.edu
Fri May 3 14:39:23 EST 1996
In article <4mcvno$m85 at net.bio.net>, Rick Wilson
<rwilson at watson.wustl.edu> wrote:
::Marc Woodland <m.p.woodland at ic.ac.uk> wrote:
::>I would love to pour gels for my 377 and leave them for longer
::>than 6 hours - like overnight ! According to the bible this is
::>not possible for two reasons (1) drying out of the very thin gels
::>and (2) hydrolysis of urea to ammonium carbonate. Presumably it
::>would be possible to prevent drying out by wrapping the top and
::>bottom of the gel in clingfilm. But if the gel is placed in a
::>cold room is the rate of urea hydrolysis slowed sufficiently to
::>ensure a good gel ? Does anyone have any ideas, comments, or
::>experience ? If so please let me know.
::When you don't like the way things are, start your own religion. We
::routinely pour gels on Friday and use them on Saturday and Sunday. The
::key is to wrap both ends with a bit of Saran Wrap so they don't dry out.
::We leave them at room temperature. Urea hydrolysis does not seem to be
::a problem. All gels are currently made with pre-made mix from either
::Amresco or Sooner Scientific.
I agree with Rick. We use LongRanger gels with 6M urea. We cover both
ends with a little saran wrap and leave them overnight for the next
morning. At present, we do not leave the gels sit longer than ~18 hours.
BTW, putting the gels at 4 degrees might cause the urea to crytalize out.
"People are DNA's way of making more DNA."(Edward O. Wilson, 1975)
\ / \ \ / \ \ / \ \ / George Mayhew \ / \ \ / \ \ / \ \
\ /\ \ /\ \ E. coli Genome Sequencing Project \ /\ \ /\ \
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