STRATEGIC FOR BIOTECH COMPANIES (Re: loading gels - what to use?)
kraev at bc.biol.ethz.ch
Mon Nov 4 10:49:31 EST 1996
In article <55ivc0$n4a at net.bio.net>, Gabriel Dorado <bb1dopeg at uco.es> wrote:
> "L.T.Hollingsworth" <hollingsworth at immunex.com> wrote:
> >Lab ergonomics is a hot topic today.
> >What alternatives are being used to load gels?
> >I have seen that ABI has recommended multichannel syringes with the XL
> >upgrade to 48 wells. I'd appreciate hearing about your experience
> >using this system.
> >We are currently loading 36-well combs on the 377 and 373 with Gilson
> >pipets and are finding wrist and fatigue problems...especially by the
> >fourth gel of the day.
> Here is an idea for BioTech Companies out there to release a new approach
> to gel loading and make lots of money. We have a 373Stretch and a 377 from
> PE/AB and loading is certainly one of the most tedious steps in "automated"
> sequencing (sharks tooth combs) and --mainly-- GeneScan analysis (plain
> combs). With the brand new XL upgrades, these problems have become even
> more significant. Now, imagine you can develop a new kind of comb. It is
> made of a special absorbent material slightly positively charged. It looks
> like a standard plain tooth comb, yet you cast the gel as if you were going
> to work with a sharks-tooth one. How does it work? You simply add a few
> Now, it is just a matter for the R&D Department of BioTech companies
> (including PE/ABI !) to search for such "wonder" material. I am sure there
> is such a product. Or else you could synthesize and patent it. You see, you
> will be able to make money licensing it to... Pharmacia and Li-Cor... :-)
Uhu, it is exactly what I have been experimenting with lately on a LI-COR
I have got some cellulose acetate samples from Scheicher-Schuell and made
them. Now, I realize it is not a work for person in my position. You need to try
many types of material, cut combs with small wells manually, etc etc.
However, I found that one can add something into the reaction ( before
cycling) to make it loadable
and, in fact, cycle sequencing reaction does not need any formamide ( I
although not with thousands of samples). Cycling in 10% DMSO plus cresol red
should make it, for example. You just need to make a special PCR
you discharge right onto the gel... :-)
It is not the reason for joking, since it is the gel loading and well
makes "automatic" sequencing only "automated", not more. And indeed it would
be advantageous for machines like LI-COR or ALF, since PE already sells
which loads automatically, only charges 20 Francs for every reaction...
Happy loading, the 8-channel syringe is not too bad for the moment.
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch
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