BAC/Sequencing

Perry d'Obrenan Perry.DObrenan at UAlberta.CA
Tue Nov 19 16:10:38 EST 1996


We have just unsuccessfully tried to sequence a ~150Kb insert in
pVelobAC11, using the PE/ABI FS dyeterminator chemistry. We used ~10ug
template and ~6.4 pmol of SP6 or T7 primers and 5% DMSO in the standard
cycle sequencing reaction mix. There was no evidence of any extension
products nor priming. If anyone has any useful suggestions I would be most
appreciative. Thanx in advance.




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