Terminator residues.

Susan Thomas st11111 at ggr.co.uk
Thu Oct 24 10:57:03 EST 1996


I purify my sequence reactions by ethanol precipitation, adding 1/10th 
vol sodium acetate (3M, pH 5.5) + 2.5 vols ethanol, both at room temp. 
After mixing, leave on ice for no longer than 10mins - I have found large 
dye blobs result if samples are left for longer than 10mins or if too 
much ethanol has been added. 

I hope this of some help.


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