ABI questions

Nelson Nelson
Wed Apr 2 16:22:02 EST 1997


I too sometimes use half volume reactions with our ABI 373Stretch 
machine.  A
couple of hints though:  If the template quality is variable (as ours 
are) the
results can be dissapointing.  The read length is usually shorter as 
well. 
Try using the halfTERM reagent from GENPAK, it's really cheap, it allows 
you
to use half of the enzyme amount and it greatly improves the read length. 
 We
usually get over 800 bases when using the halfTERM reagent.

Tom
BMS PRI
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Remember, Tom's opinions are only his and not his employer's ;-)
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