Thu Apr 10 16:12:39 EST 1997
Yes, I have seen this happen with a clone which contained a large inverted
repeat. The sequence read through the vector sequence to the cloning
skipped over the inverted repeat and continued on the other side.
Peter J. Myler, Ph.D.
Staff Scientist Assistant Professor
Seattle Biomedical Research Institute Department of Pathobiology
4 Nickerson Street University of
Seattle, WA 98109-1651 Seattle, WA 98195
e-mail: mylerpj at sbri.org mylerpj at u.washington.edu
charlie demma; demma at welchlink.welch.jhu.edu wrote in article
<5ibmdq$pt at net.bio.net>...
> We did some sequencing for someone on an ABI 377. He was absolutely
> positive that his insert was in his plasmid but the insert was missing
> from his electropherogram. Has anyone ever seen a situation where the
> polymerase will skip a region and continue on to give a nice sequence?
> Charlie Demma
> Demma at welchlink.welch.jhu.edu
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