ABI PMT setting

Michael Michael
Mon Apr 14 17:12:18 EST 1997


We are having a problem with our 373 upgrade sequencer.  The PMT value
must be set at around 800 to get a blue line (y value) of around 700.  The
laser and filter were just replaced (ABI thought that this was the
problem).  The machine was just inherited from a lab that shut down so we
do not know the history.  The standardish PMT setting on other machines is
usually around 530-560.  Has anyone had this problem in the past and know
what should be done to correct it?  I have heard of these high settings
when using the base-sprinter but never when doing a normal overnight run.

Thanks in advance for any help.

Mike

-- 
Michael C. Gorry
University of Pittsburgh
Center for Genomic Sciences
mcgorry at med.pitt.edu



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