ABI PMT settings
Tue Apr 15 08:21:04 EST 1997
Michael C. Gorry Wrote:
We are having a problem with our 373 upgrade sequencer. The PMT value
must be set at around 800 to get a blue line (y value) of around 700. The
laser and filter were just replaced (ABI thought that this was the
problem). The machine was just inherited from a lab that shut down so we
do not know the history. The standardish PMT setting on other machines is
usually around 530-560. Has anyone had this problem in the past and know
what should be done to correct it? I have heard of these high settings
when using the base-sprinter but never when doing a normal overnight run.
Thanks in advance for any help.
Michael C. Gorry
Dear Newsgroupers ,
We have just had one of our 373's upgraded to the XL ( 64 lane ) with
initial problems with getting the PMT to a satisfactory level . The scan
line were just not getting to the ideal 750 - 1000 level . A variety of
things were attempted but the final releif was getting a new
Photomultiplier tube fitted . I am not sure if you have had a recent
upgrade but if you have check into this .
We have also spotted a bug where if you change the scan mode after a
plate check has been performed the machine must be completely reset to
get it to communicate with the 373 itself . Something which is a minor
hassle to say the least .
Hope this is of some help ,
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