DD-PCR sequencing etc
Tue Aug 19 11:31:22 EST 1997
has any one worked with custom dye primers ?
any one out there doing direct (without subcloning) sequencing
of DD-PCR fragment ? We would very much like to hear from you on -
1) what is the typical success rate ?
2) what degree of automation and what throughput can you build ?
3) how do you quantitate / evaluate amplification products ?
4) what is the best sequencing strategy - nested primer ? custome
dye primer labeling ?
any information is appreciated.
Simon Dai /Gene Logic Inc.
More information about the Autoseq