DD-PCR sequencing etc

simon simon
Tue Aug 19 11:31:22 EST 1997


Hi, everyone

has any one worked with custom dye primers ?


any one out there doing direct (without subcloning) sequencing 
of DD-PCR fragment ?  We would very much like to hear from you on -
1) what is the typical success rate ?
2) what degree of automation and what throughput can you build ?
3) how do you quantitate / evaluate amplification products ?
4) what is the best sequencing strategy - nested primer ? custome 
dye primer labeling ? 

any information is appreciated.

Simon Dai /Gene Logic Inc.



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