BigDyes and 373 Sequencers

[Michael Lassner]

No question about it. The new dyes are better.  If you switch, you won't
regret not being able to use the old dyes.


> 
> Hi all
> 
> ABI have produced a filter wheel which can be fitted to a 373A sequencer
> to allow it to take advantage of the new generation of dyes (which 377
> users can automatically use).  These are the dRhodamine terminators and
> the BigDye primers and terminators.
> 
> For people who use these new dyes, are they that much better than the
> older ones?
> 
> For 373A users changing ther filter wheel means you cannot use the older
> dyes anymore.  It would also prevent sequencers from using other
> manufacturers dye kits (Amersham for example).
> 
> Has anybody using a 373A switched to the new filter wheel? and do you
> find you get better quality data?
> 
> Thanks in advance.
> 
> Mick
> 
> Dr Mick Jones, Lecturer in Molecular Virology
> Department of Infectious Diseases, Imperial College School of Medicine
> Hammersmith Hospital, Du Cane Road, London, W12 0NN
> Tel:  0181-383-3328;  Fax:  0181-383-2299
> email:  mdjones at ic.ac.uk
> ----------------------------------------------------------------
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