cycle sequencing with Pharmacia's ALF

[Ari-Matti]

In article <5dg5g6$ff9 at net.bio.net> Jochen Thur, Jochen.Thur at Uni-Koeln.DE 
writes:
>From: Jochen Thur, Jochen.Thur at Uni-Koeln.DE
>Subject: cycle sequencing with Pharmacia's ALF
>Date: 7 Feb 1997 13:06:14 -0800

>Hi ALF Sequencers:
>Does anyone know a protocol to use fluorescein-labelled primers in cycle 
>sequencing reactions for the ALF system ?

We use the Thermo Sequenase kits from Amersham, and they perform 
consistently well. So well in fact, that we only use T7 in "special 
occasions".(The price is not that bad to start with, and it's possible 
to use less reaction mixture than specified.)

Ari-Matti