Sequencing through poly A region

Juergen Juergen
Wed Feb 19 16:44:48 EST 1997


In article <5ct2rc$f64 at net.bio.net>, Muhammad A. Lodhi, lodhi at cshl.org 
wrote:

> experience of sequencing
> through a region of ~22 As

get out the good old DMS, hydrazine, piperidine etc. and sequence it the
Maxam-Gilbert way.  I had no trouble to sequence through a stretch of 22
A-residues in the rat BC1 RNA genomic sequence.  PCR is often the cause of
trouble, since the polymerase stutters through homopolymers.

Good luck,
             Juergen

-- 
                                          usual disclaimers
Juergen Brosius
Inst. f. eXp. Pathol.
University of Muenster
Von-Esmarch-Str. 56
D-48149 Muenster, Germany

Fax: +49  251  835 8512
Tel: +49  251  835 8511



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