Sequencing through poly A region
Wed Feb 19 16:44:48 EST 1997
In article <5ct2rc$f64 at net.bio.net>, Muhammad A. Lodhi, lodhi at cshl.org
> experience of sequencing
> through a region of ~22 As
get out the good old DMS, hydrazine, piperidine etc. and sequence it the
Maxam-Gilbert way. I had no trouble to sequence through a stretch of 22
A-residues in the rat BC1 RNA genomic sequence. PCR is often the cause of
trouble, since the polymerase stutters through homopolymers.
Inst. f. eXp. Pathol.
University of Muenster
D-48149 Muenster, Germany
Fax: +49 251 835 8512
Tel: +49 251 835 8511
More information about the Autoseq