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compressed lanes

C.Guihal at rbge.org.uk C.Guihal at rbge.org.uk
Thu Feb 20 09:10:48 EST 1997


Date:          Thu, 20 Feb 1997 8:47:38 BST
Subject:       Re: Compressed lanes--too many salts?
Priority: normal
Good morning,

well ourselves, we are using a mix 70% ethanol/ 0.5mM MgCl2 (74uL added 
to the 
20uL reaction), and after centrifugation, and complete removal of the 
supernatant, we are able to read the sequence from the 10th nucleotide.
As we load samples in two times we don't have any problem to track each 
lane.

hope that will help,
Caroline GUIHAL
Scientific and technical services Department
Royal Botanic Garden Edinburgh
20A inverleith Row
Edinburgh EH3 5LR
Scotland, UK
Tel: 0131 552 7171
Fax: 0131 552 0382
e.mail: C.GUIHAL at rbge.org.uk



Caroline GUIHAL
Scientific and technical services Department
Royal Botanic Garden Edinburgh
20A inverleith Row
Edinburgh EH3 5LR
Scotland, UK
Tel: 0131 552 7171
Fax: 0131 552 0382
e.mail: C.GUIHAL at rbge.org.uk



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