Multiplexing of AFLP's on the ABI 377
Mon Feb 24 10:00:41 EST 1997
Last year we've started with fragmentanalysis( AFLP's ) on the ABI Prism
For the moment we run our samples as follow :
- one sample (one primercombination)
- GS-500 ROX internal lane standard
If we use 3 samples with the GS-500 ROX we loose a lot of signal
with the TAMRA dye.
So for the moment we can't take advantage of the multiplex strategy.
Does anyone has experience with the multiplexing (4 dyes / lane) of AFLP's
on the ABI Prism 377 DNA Sequencer?
RvP- Rijksstation voor Plantenveredeling
Plant Breeding Station
Mail : RvP at pophost.eunet.be
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