does anyone have any suggestions for sequencing G-C rich
plasmids ? : We have tried all the usual tricks, including doubling the
amount of taq & primer ( sequencing is based on diterminator chemistry,
utilising an ABI 377 ), increasing the denaturation temp. and the
denaturation time and finally denaturing the plasmid itself prior to
cycle sequencing. Is there anything left to try ?
Laurence S. Hall.
School of Biological Sciences,
2.237 - Biomolecules,
Division of Biochemistry,
University of Manchester,
Manchester M13 9PT.
Tel. : +44 (0)161 275 6916. E-mail :
LHALL at fs2.scg.man.ac.uk
Fax : +44 (0)161 5082.