Loss of T signal
Thu Jan 9 12:26:17 EST 1997
I seem to have lost all my T's!
The first run after Christmas and a beautiful black background should
have made me suspicious.
The gel image looked good. But the base called trace did not
have any T's in it. This was ABI's template and primer. When I
looked for the signal strength the T signal was just about the same as
the others. Some say they were a little too high, between 500 and
Someone else in the lab has just run another set of samples and
again there does not seem to be any T's called?
The number on the side of the tube of dye terminator mix is
361405010, this does not seem to be the same as the lot number on the
side of the box though?
We are trying again with different batches of dye terminators.
We are useing 36 cm plates with Anachem's pre mixed gel (that we have
frozen) along with Anachem's pre mixed 5x TBE. The machine is a 377
that we've had since September 1996.
Can anyone tell me I'm not alone with this problem, and whats
more does anyone know how to make it go away? Even better does anyone
know what has caused this to (apparenlty) happen so suddenly. Things
were going fine (well comparatively) before Christmas.
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