Dye Primers & Heterozygotes

Patricia Patricia
Mon Jan 20 15:52:23 EST 1997


Dear Shaun,

We may be onto something here.  2 people have come to my core lab with
Amersham's ET primers and I've had the same problem...blue overlay on the
gel image where those samples were loaded.  I mean, it even looks as 
though
the signal strength is weak underneath the blue, but when you print out 
the
electropherograms, everything looks in order, even great.  My worry is
this: It's impossible to track these blue lanes.  If any tracking issue
arose, I'd be in trouble.  Has anyone else used these products? The matrix
files looked fine and the samples were user-prepared.  Most importantly, 
am
I doing something WRONG?!!

                                                --Patricia

Patricia Thomas
Washington Univ. School of Medicine
Department of Molecular Biology & Pharmacology
660 South Euclid
Box 8103
St. Louis, MO  63110
thomas at pharmdec.wustl.edu
Lab address:
        Dr. Kerry Kornfeld's lab
        Cancer Research Building, 3rd floor
        (314)747-2003 or 747-2004





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