ABRF DNA sequencing tutorial

Harold Harold
Mon Jan 20 09:15:35 EST 1997


HI,

I would appreciate a note directly to me and not posted to the groups:

 1. Who will be attending the tutorial, it will help in preparing enough
handouts.

 2. Specific questions you want addressed.  Don't be afraid to ask if you
are a beginner and that's also why you should respond directly to me and
not to the whole group.

 3. Also some indication of your level of expertise.

Bruce Roe does not need to answer the last question.

We will address reaction volume, 5,10 and 20 ul.  Examples will be in the
handout.

The merits of LongRanger, PagePlus, 29:1 and 37.5:1 acrylamide at the 2x
and 4x speed.  Examples of results will be included in the handouts.

Optimum template amount in the reactions.

One of the panel is a Licor user.

Three of us have 377's, and two of us are using the 3.0 software.

Hal


Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University
Ames, IA 50011-3260				  hhills at iastate.edu
http://biotech.zool.iastate.edu/Biotech/Facilities/DSSF/homepage.html





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