abi377

MAL at CALVIN.BWH.HARVARD.EDU MAL at CALVIN.BWH.HARVARD.EDU
Tue Jan 21 10:29:21 EST 1997


  
  dear fellow sequencers,
  we , the Brigham and Women's Hopital seqeuncing facility in Bosotn, 
have participated 
  in the abrf test  but have so far not received any results. We are very 
  interested in the experience of other 377 users with the 4x run mode. 
what is 
  this abrf tutorial about? i seem to have missed some of the 
conversation here.
   we routinely use 5 ul rxns, ETOH ppthn  and LR. in the near future we 
will start to use 
  our 377 for genotyping. has anybody experience with multiplexing etc...
we are are escially interested in any information regarding multiplex pcr 
and 
how people test and optimize their conditions.
also, has anybody successfully used the 48 lanes on the 377 using 4x run 
mode 
either for sequencing or genotyping?

  thank you

  sincerely
  Min Ae Lee,M.D.
  Fellow in CArdiology
  Brigham and Women's Hospital
  20 Shattuck Street
  Bosotn,MA 02115
  tel 617-732 8174
  fax 617-268 6830
  




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