Peak stretching around 200bp

Dr Dr
Wed Jan 29 10:50:55 EST 1997


Dear Sequencers,
                we have been seeing stretching of peaks from around 190
to 210 bp across the gel on a 377 with resulting loss of resolution. The
peaks return to their normal width after this so it seems to be a
localised problem at around these fragment sizes. We are doing 3.5 hour
runs on 36cm plates using dye-terminator chemistry with the ABI kit on
double stranded plasmid DNA. Has anyone seen this and know what might be
causing it? 
Thanks in advance
Tim Sawbridge

T.Sawbridge at forbio.com.au



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