Dir. Seq. of small PCR frag.
Mon Jul 21 10:10:18 EST 1997
Are you sure you're getting too much signal? Is the gel lane really
If you analyse a short fragment with the standard settings maybe the low
after the end of the template would cause the actual sequence to be
a huge trace, apparently too strong to read.
I ask because very strong signal shouldn't really cause problems - you
load less of the reaction!
Hope this helps,
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