Dir. Seq. of small PCR frag.

Anthony Anthony
Mon Jul 21 10:10:18 EST 1997


Are you sure you're getting too much signal?  Is the gel lane really
bright?
 If you analyse a short fragment with the standard settings maybe the low
signal
 after the end of the template would cause the actual sequence to be
displayed as
 a huge trace, apparently too strong to read.
 
 
 I ask because very strong signal shouldn't really cause problems - you
could just
 load less of the reaction!
 
 Hope this helps,
 
 Anthony



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