Dir. Seq. of small PCR frag.
Tue Jul 22 10:42:09 EST 1997
Thank you to everyone who replied to my posting re: direct sequencing of a
small PCR product and the 'high signal' problem which prompted my post. I
recieved many helpful suggestions, both via e-mail and replies posted
The solution in fact turned out to be rather simple: a combination of
the loading amount and setting the read endpoint within a few bases of the
fragment ending. I really appreciate the time and effort it took for
to help me out, and I must say that having this resource available is
extremely valuable to everyone in the community.
Charles Warren, student
Molecular Biomarker Laboratory
DEH, University of Washington
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