Dye blobs

Kari Kari
Fri Jun 6 09:54:54 EST 1997

We have recently had lots of trouble with big yellow dye blobs at nt
20-40.  I understand that these are unincorporated nucleotides, which
have not been removed by the spin columns.  We have tried AGTC columns,
washed AGTC columns, Pharmacia AutoSeq columns and today are trying
repoured Centrisep columns.  None of these has had any effect on the dye
blobs so far.  We run half reactions and bring them up to 20 ul before
loading the columns.  Any ideas?

Kari L. Ohlsen Ph. D.
Microchemical Core Facility
San Diego State University
San Diego, CA  92182

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