Gel Blobs on ABI 377's

Tony Tony
Wed Mar 5 09:01:40 EST 1997


 Anyone know what causes a huge blue blob that appears in the gel image
during a sequence run on an ABI 377 using a 4% acrylamide gel?  I'm sure
it's a gel problem of somekind but I don't know what.  The plate checks
usually look fine and during the run about 15-45 minutes into the run
(4XA) the blob appears and will ruin between 5 and 30 reads (out of 36
lanes).  Anyone else experienced this?

-Tony




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