No Sequence

GMEACHAM at bmg.bhs.uab.edu GMEACHAM at bmg.bhs.uab.edu
Tue May 27 12:14:26 EST 1997


I have sent a plasmid to be sequenced using a Perkin Elmer automated 
system. They use the ABI PRISM dye terminator cycle sequencing kit.
 I can PCR the sequence out (with low efficiency), but am unable to get 
any sequence using the components above. The plasmid and ORF isn't GC 
rich and my primers are desalted/deprotected. Any suggestions would be 
helpful. Thanks in advance.




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