BAC-end sequencing with BIG dyes. Best protocol??

Stan L Lilleberg, Ph.D. stanl at lexgen.com
Tue Nov 11 05:02:51 EST 1997


Okay, we all have had plenty of time to try this.  So what is the best
protocol for BAC-end sequencing with the ABI BIG dye terminators??

Template and Primer concentration?
Cycle Profile?
Thermofidelase?
DMSO?




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