A little tip - some of you may know this I did'nt I had to
ring ABI. To stop you having to ring ABI at 1650 on a Friday
when they should be getting the "tinnies" out here we
You've installed the new software all seems to be O.K. your
gel is loaded you go to the start icon and and error message
comes up "Flow detected with pump off"
DO NOT PANIC
Go to "Window" and open up Mannual control. Select "Pump On"
and for value insert 51, for range 0-60 then execute. Then
select "Pump Off". repeat this cycle a few times then try to
restart your run. You may need to do this more than once. I
had three gels run under these conditions the last time I think
I mannually 'switched the pump on/off about 15x before it ran.
If this doesn't work you could reinstall the old software or
BURST INTO TEARS and ring your engineer for some counselling.
M.P. Woodland Ph.D.
DNA Sequencing Core Facility Manager,
Dept. Of Biochenistry,
Imperial College of Science, Technology & Medicine,
London SW7 2AY.
Phone (44) 0171 595 5307 (direct)
EMail m.p.woodland at ic.ac.uk