96-well preping protocols please.

Kevin Clark kevin at hammer.imm.ox.ac.uk
Tue Oct 21 07:41:13 EST 1997


Hello,

We are going to be starting a core facility soon where I work and so I'll
be moving up from running 48 samples a day to 100-150.

I currently do my M13 preps using a peg/phenol extraction which is a little
time consuming but works beautifully 99.9% of the time. With the facility
needing more samples I need to move up to 96 well format preping methods
certainly for my M13's and possibly also for pcr products and
post-sequencing reaction clean ups.

Does anyone have any suggestions? I may also have access to a Biomek 2000
so methods for that would be welcome too.

Regards

Kev

Kevin Clark                    |If a dolphin will jump out
kevin at hammer.imm.ox.ac.uk      |of the water for a fish,
Institute of Molecular Medicine|imagine what it would do
Oxford,UK.                     |for a bag of chips.






More information about the Autoseq mailing list