TBE

[Harold G. Hills]

In my experience, when making TBE,it is important to thoroughly dissolve
the Tris and EDTA before adding the Boric acid.  If the Tris and EDTA are
stirred for 15-20 minutes before adding in the Boric acid, it will almost
never precipitate.

A much easier solution is to switch to Tris Taurine buffer made as a 20X
stock that never precipitates, does not need to be autoclaved and because
it is a 20x stock you make it half as often.  Remember to add 1/2 amount to
your gels because it is 20 x and not 10 x.  At the ABRF URL below there is
a recipe in the Taurine buffer html.  Thank Laura Livingstone of UNC for
working this out.

Hal

Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.