TBE

Harold G. Hills hhills at iastate.edu
Sun Oct 26 10:58:44 EST 1997


In my experience, when making TBE,it is important to thoroughly dissolve
the Tris and EDTA before adding the Boric acid.  If the Tris and EDTA are
stirred for 15-20 minutes before adding in the Boric acid, it will almost
never precipitate.

A much easier solution is to switch to Tris Taurine buffer made as a 20X
stock that never precipitates, does not need to be autoclaved and because
it is a 20x stock you make it half as often.  Remember to add 1/2 amount to
your gels because it is 20 x and not 10 x.  At the ABRF URL below there is
a recipe in the Taurine buffer html.  Thank Laura Livingstone of UNC for
working this out.

Hal

Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.






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