1x TBE for running gels

Shaun Tyler styler at hwc.ca
Thu Oct 30 04:40:43 EST 1997


I have always found that 10x TBE will eventually precipitate -- so will
5x.  What I normally do is to autoclave the 10x buffer.  This seems to
give it a longer shelf life before precipitating.  As soon as you notice
it precipitating just autoclave it again.  This puts it back into
solution and doesn=92t seem to affect the buffer at all.

Shaun Tyler
DNA Core Facility
Laboratory Centre for Disease Control
Health Canada

Ph#:  (613) 941-6441
FAX#: (613) 957-1358

E-mail:  styler at hpb.hwc.ca




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